Granada Medium

[所属分类:培养基配方] [发布时间:2021-3-19] [发布人: ] [阅读次数:] [返回]

Granada Medium

培养基配方

Composition per liter:
Starch, soluble...........................................................................150.0g
Proteose peptone No. 3...............................................................38.0g
NaCl..............................................................................................3.0g
Trimethoprim lactate.................................................................0.015g
Sodium phosphate
buffer (0.06M, pH 7.4) ....................................................900.0mL
Horse serum, coagulated........................................................100.0mL
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add proteose peptone No. 3 and NaCl to 200.0mL of sodium phosphate buffer and bring to boiling. Add 400.0mL of cold sodium phosphate buffer, starch, and trimethoprim lactate. Mix thoroughly. Bring volume to 900.0mL with sodium phosphate buffer. Gently heat while stirring in a boiling water bath for exactly 20 min. Do not autoclave. Cool to 90°–95°C. Add horse serum. Mix thoroughly. Cool to 60°–65°C while stirring. Pour into sterile Petri dishes. Medium will solidify in 2–3 hr.
Use: For the early selective isolation and cultivation of Group B streptococci from clinical specimens.