Group A Selective Strep Agar with Sheep Blood

[所属分类:培养基配方] [发布时间:2021-3-19] [发布人: ] [阅读次数:] [返回]

Group A Selective Strep Agar with Sheep Blood

培养基配方

Composition per liter:
Pancreatic digest of casein..........................................................14.5g
Agar ............................................................................................14.0g

NaCl..............................................................................................5.0g

Papaic digest of soybean meal......................................................5.0g
Sheep blood .............................................................................50.0mL
Growth factor solution.............................................................10.0mL
Selective agents solution..........................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Growth Factor Solution:
Composition per 10.0mL:
Growth factors, BBL ....................................................................1.5g
Preparation of Growth Factor Solution: Add growth factors to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Selective Agents Solution:
Composition per 10.0mL:
Selective agents ........................................................................0.042g
Preparation of Selective Agents Solution: Add selective agents to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except sheep blood, growth factor solution, and selective agents solution, to distilled/deionized water and bring volume to 930.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of sheep blood, 10.0mL of sterile growth factor solution, and 10.0mL of sterile selective agents solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the selective cultivation and primary isolation of group A streptococci, especially Streptococcus pyogenes, from clinical specimens.