GS Medium

[所属分类:培养基配方] [发布时间:2021-3-19] [发布人: ] [阅读次数:] [返回]

GS Medium

培养基配方

Composition per liter:
Morpholinopropane sulfonic acid...............................................10.0g
Yeast extract..................................................................................6.0g
Glucose.........................................................................................5.0g
Urea...............................................................................................2.0g
L -Cysteine·HCl .............................................................................1.0g
K 2 HPO 4 ·3H 2 O ..............................................................................1.0g
KH 2 PO 4 .........................................................................................0.5g
MgCl 2 ·6H 2 O .................................................................................0.5g
CaCl 2 ·2H 2 O ................................................................................0.05g
FeSO 4 ·7H 2 O.............................................................................1.25mg
Resazurin...................................................................................1.0mg
pH 7.2 ± 0.2 at 25°C
Glucose Solution:
Composition per 20.0mL:
Glucose.........................................................................................2.0g
Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize. Sparge with 100% N 2 .
Preparation of Medium: Add components, except glucose solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Sparge with 100% N 2 . Autoclave for 25 min at 15 psi pressure–121°C. Aseptically and anaerobically add 20.0mL of sterile glucose solution. Mix thoroughly.

Use: For the cultivation and maintenance of Clostridium thermocellum.