Haemophilus Agar

[所属分类:培养基配方] [发布时间:2021-3-23] [发布人: ] [阅读次数:] [返回]

Haemophilus Agar

培养基配方

Composition per 1010.0mL:
Beef heart, infusion from..........................................................250.0g
Calf brain, infusion from ..........................................................200.0g
Agar ............................................................................................13.5g
Proteose peptone.........................................................................10.0g
NaCl..............................................................................................5.0g
Na 2 HPO 4 ·12H 2 O...........................................................................2.5g
Glucose .........................................................................................2.0g
β-NADH .......................................................................................1.0g
L -Cysteine·HCl..............................................................................0.5g
Chicken serum, inactivated......................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components, except β-NADH,  L - cysteine·HCl, and chicken serum, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 1.0g of β-NADH, 0.5g of  L - cysteine·HCl, and 10.0mL of chicken serum. Mix thoroughly. Pour intosterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Haemophilus paragallinarum.