Haemophilus ducreyi Medium

[所属分类:培养基配方] [发布时间:2021-3-23] [发布人: ] [阅读次数:] [返回]

Haemophilus ducreyi Medium

培养基配方

Composition per liter:
Columbia blood agar base......................................................675.0mL
Rabbit blood...........................................................................300.0mL
Fresh yeast extract solution......................................................25.0mL
pH 6.5–7.0 at 25°C
Columbia Blood Agar Base:
Composition per 675.0mL:
Agar ............................................................................................15.0g
Pantone........................................................................................10.0g
Bitone..........................................................................................10.0g
NaCl..............................................................................................5.0g
Tryptic digest of beef heart...........................................................3.0g
Cornstarch.....................................................................................1.0g
Preparation of Columbia Blood Agar Base: Add components to distilled/deionized water and bring volume to 675.0mL. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Fresh Yeast Extract Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free......................................25.0g
Preparation of Fresh Yeast Extract Solution : Add the live Baker’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90 min at 15 psi pressure–121°C. Allow to stand. Remove supernatant solution. Adjust pH to 6.6–6.8. Filter sterilize.
Preparation of Medium:  To 675.0mL of cooled, sterile Columbia blood agar base, aseptically add rabbit blood and sterile fresh yeast extract solution. Aseptically adjust pH to 6.5–7.0.
Use:  For the cultivation and maintenance of Haemophilus ducreyi.