Haemophilus influenzae Defined Medium MI-Cit

[所属分类:培养基配方] [发布时间:2021-3-23] [发布人: ] [阅读次数:] [返回]

Haemophilus influenzae Defined Medium MI-Cit

培养基配方

Composition per liter:
NaCl..............................................................................................5.8g
K 2 HPO 4 .........................................................................................3.5g
Glycerol ........................................................................................3.0g
KH 2 PO 4 .........................................................................................2.7g
Inosine...........................................................................................2.0g
L- Glutamic acid.............................................................................1.3g
K 2 SO 4 ............................................................................................1.0g
Sodium lactate...............................................................................0.8g
L- Aspartic acid ..............................................................................0.5g
Nitrilotriethanol ............................................................................0.4g
L- Leucine.......................................................................................0.3g
L- Cystine .......................................................................................0.2g
MgCl 2 ............................................................................................0.2g
L- Tyrosine......................................................................................0.2g
Citrulline.....................................................................................0.15g
L- Methionine.................................................................................0.1g
L- Serine .........................................................................................0.1g
L- Lysine.......................................................................................0.05g
Glycine........................................................................................0.03g
CaCl 2 .........................................................................................0.022g
Hypoxanthine..............................................................................0.02g

Polyvinyl alcohol........................................................................0.02g

Tween™ 80.................................................................................0.02g
Hemin .........................................................................................0.01g
L- Histidine...................................................................................0.01g
Calcium pantothenate ................................................................4.0mg
Ethylenediaminetetraacetate......................................................4.0mg
Nicotinamide adenine dinucleotide ...........................................4.0mg
Thiamine....................................................................................4.0mg
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.
Use: For the cultivation of Haemophilus influenzae in a chemically defined medium.