Haemophilus Medium (DSMZ Medium 804)

[所属分类:培养基配方] [发布时间:2021-3-23] [发布人: ] [阅读次数:] [返回]

Haemophilus Medium

(DSMZ Medium 804)

培养基配方

Composition per liter:
Acid hydrolysate of casein..........................................................31.5g
Beef extract...................................................................................5.4g
Yeast extract..................................................................................5.0g
Starch............................................................................................2.7g
NAD solution.............................................................................1.0mL
Hemin solution...........................................................................1.0mL
pH 7.3 ± 0.1 at 25°C
NAD Solution:
Composition per 10.0mL:
NAD.......................................................................................150.0mg
Preparation of NAD Solution:  Add NAD to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Hemin Solution:
Composition per 10.0mL:
Hemin ....................................................................................150.0mg
NaOH (1N solution)...................................................................2.0mL
Preparation of Hemin Solution:  Add hemin to 2.0mL of 1N NaOH solution. Mix thoroughly. Bring volume to 10.0mL with distilled/deionized water. Filter sterilize.
Preparation of Medium: Add components, except hemin solution and NAD solution, to distilled/deionized water and bring to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 10 min at 10 psi pressure–115°C. Cool to 25°C. Aseptically add 1.0mL sterile NAD solution and 1.0mL sterile hemin solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Haemophilus influenzae.