Haemophilus somnus Agar

[所属分类:培养基配方] [发布时间:2021-3-23] [发布人: ] [阅读次数:] [返回]

Haemophilus somnus Agar

培养基配方

Composition per liter:
Agar ............................................................................................20.0g
Tryptose ......................................................................................10.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................3.0g
Sheep blood, defibrinated......................................................100.0mL
IsoVitaleX ® enrichment solution.............................................10.0mL
L -Cysteine·HCl solution.............................................................5.0mL
L -Cysteine·HCl Solution:
Composition per 10.0mL:
L -Cysteine·HCl..............................................................................1.0g
Preparation of  L -Cysteine·HCl Solution:  Dissolve 1.0g of  L - cysteine·HCl in distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Warm to 50°C.
IsoVitaleX ® Enrichment Solution:
Composition per liter:
Glucose .....................................................................................100.0g
L- Cysteine·HCl............................................................................25.9g
L- Glutamine.................................................................................10.0g
Adenine.........................................................................................1.0g
Thiamine pyrophosphate...............................................................0.1g
Vitamin B 12 ...................................................................................0.1g
Guanine·HCl...............................................................................0.03g
Fe(NO 3 ) 3 ·9H 2 O...........................................................................0.02g
p-Aminobenzoic acid................................................................0.013g
Thiamine·HCl ...........................................................................0.003g
Preparation of IsoVitaleX ® Enrichment: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Warm to 50°C.
Preparation of Medium: Add components, except sheep blood, IsoVitaleX ® enrichment solution, and  L -cysteine·HCl solution, to distilled/deionized water and bring volume to 895.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Warm defibrinated sheep blood to 50°C. Aseptically add 100.0mL of sterile defibrinated sheep blood, 10.0mL of sterileIsoVitaleX ® enrichment solution, and 5.0mL of sterile  L -cysteine·HCl solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Haemophilus somnus.