Half Fraser Broth without Ferric Ammonium Citrate

[所属分类:培养基配方] [发布时间:2021-3-24] [发布人: ] [阅读次数:] [返回]

Half Fraser Broth without Ferric Ammonium Citrate

培养基配方

Composition per liter:
NaCl............................................................................................20.0g
Na 2 HPO 4 .....................................................................................12.0g
Proteose peptone...........................................................................5.0g
Tryptone........................................................................................5.0g
Lab Lemco powder.......................................................................5.0g
LiCl...............................................................................................3.0g
KH 2 PO 4 .......................................................................................1.35g
Esculin..........................................................................................1.0g
Half Fraser supplement solution without
ferric ammonium citrate....................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid Unipath.

Half Fraser Supplement Solution without Ferric Ammonium Citrate:

Composition per 10.0mL:
Acriflavine·HCl ........................................................................0.125g
Nalidixic acid..............................................................................0.05g
Ethanol.......................................................................................5.0mL
Preparation of Half Fraser Supplement Solution without Ferric Ammonium Citrate: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except half Fraser supplement solution without ferric ammonium citrate, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile Half Fraser supplement solution without ferric ammonium citrate. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the isolation of Listeria species from food and environmental specimens. A primary selective enrichment broth for Listeria spp. A pre-supplemented primary selective enrichment broth for Listeria spp.