Haliscomenobacter hydrossis Medium (LMG Medium 154)

[所属分类:培养基配方] [发布时间:2021-3-24] [发布人:王亚萍] [阅读次数:] [返回]

Haliscomenobacter hydrossis Medium

(LMG Medium 154)

培养基配方

Composition per liter:
Glutamic acid..............................................................................1.31g
MgSO 4 ·7H 2 O...........................................................................75.0mg
CaCl 2 ·2H 2 O .............................................................................50.0mg
K 2 HPO 4 ....................................................................................40.0mg
Na 2 HPO 4 ·2H 2 O........................................................................40.0mg
KH 2 PO 4 ....................................................................................27.0mg
FeCl 3 ·6H 2 O................................................................................5.0mg
MnSO 4 ·H 2 O...............................................................................3.0mg
Pancreatic digest of casein.........................................................1.7mg
NaCl...........................................................................................0.5mg
Papaic digest of soybean meal...................................................0.3mg
K 2 HPO 4 ....................................................................................0.25mg
Glucose....................................................................................0.25mg
Glucose solution......................................................................10.0mL
Vitamin solution.........................................................................1.0mL
Trace elements solution.............................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Glucose Solution:
Composition per 10.0mL:

Glucose.........................................................................................2.0g

Preparation of Glucose Solution: Add glucose to 10.0mL of distilled/deionized water. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Vitamin Solution:
Composition per 10.0mL:
Thiamine....................................................................................4.0mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution: Add Vitamin B 12 and thiamine to 10.0mL of distilled/deionized water. Mix thoroughly. Filter sterilize.
Trace Elements Solution:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
Na 2 MoO 4 ·2H 2 O .......................................................................30.0mg
MnCl 2 ·4H 2 O.............................................................................30.0mg
NiCl 2 ·6H 2 O..............................................................................20.0mg
CuCl 2 ·2H 2 O .............................................................................10.0mg
Preparation of Trace Elements Solution:  Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components, except vitamin solution and glucose solution, to 989.0mL distilled/deionized water. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 10.0mL sterile glucose solution and 1.0mL sterile vitamin solution. Mix thoroughly. Aseptically distribute to sterile tubes or flasks.
Use: For the cultivation of Haliscomenobacter hydrossis.