Haliscomenobacter Medium

[所属分类:培养基配方] [发布时间:2021-3-24] [发布人: ] [阅读次数:] [返回]

Haliscomenobacter Medium

培养基配方

Composition per liter:
Agar ............................................................................................10.0g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
Glucose .......................................................................................0.15g
CaCO 3 ...........................................................................................0.1g
KCl..............................................................................................0.05g
K 2 HPO 4 .......................................................................................0.05g
MgSO 4 ·7H 2 O..............................................................................0.05g
Ca(NO 3 ) 2 .....................................................................................0.01g
Vitamin solution.......................................................................10.0mL
Vitamin Solution:
Composition per 10.0mL:
Thiamine....................................................................................0.4mg
Vitamin B 12 ..............................................................................0.05mg
Preparation of Vitamin Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except vitamin solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile vitamin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use:  For the isolation of Haliscomenobacter species from activated sludge.