Haliscomenobacter Medium (DSM 134)

[所属分类:培养基配方] [发布时间:2021-3-24] [发布人: ] [阅读次数:] [返回]

Haliscomenobacter Medium

(DSM 134)

培养基配方

Composition per liter:
Glutamic acid..............................................................................1.31g
MgSO 4 ·7H 2 O............................................................................0.075g
CaCl 2 ·2H 2 O ................................................................................0.05g
K 2 HPO 4 .......................................................................................0.04g
Na 2 HPO 4 ·2H 2 O...........................................................................0.04g
KH 2 PO 4 .....................................................................................0.027g
FeCl 3 ·6H 2 O................................................................................5.0mg
MnSO 4 ·H 2 O...............................................................................3.0mg
Pancreatic digest of casein.........................................................1.7mg
NaCl...........................................................................................0.5mg
Papaic digest of soybean meal...................................................0.3mg
K 2 HPO 4 ....................................................................................0.25mg
Vitamin solution.......................................................................10.0mL
Glucose solution........................................................................5.0mL
Trace elements solution SL - 6.....................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Vitamin Solution:
Composition per 10.0mL:
Thiamine....................................................................................0.4mg
Vitamin B 12 ..............................................................................0.01mg
Preparation of Vitamin Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Glucose Solution:
Composition per 5.0mL:
D- Glucose......................................................................................2.0g
Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 5.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O ...............................................................................0.03g
Na 2 MoO 4 ·H 2 O............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2· 2H 2 O.................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 3.4.
Preparation of Medium: Add components, except vitamin solution and glucose solution, to distilled/deionized water and bring volume to 985.0mL. Mix thoroughly. Adjust pH to 7.5. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add sterile vitamin solution and glucose solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Haliscomenobacter hydrossis.