Haloalkaliphilic Agar

[所属分类:培养基配方] [发布时间:2021-3-24] [发布人: ] [阅读次数:] [返回]

Haloalkaliphilic Agar

培养基配方

Composition per liter:
Solution A..............................................................................900.0mL
Solution B..............................................................................100.0mL
pH 8.5–9.5 at 25°C
Solution A:
Composition per 900.0mL:
NaCl..........................................................................................200.0g
Agar ............................................................................................25.0g
Yeast extract................................................................................10.0g
Casamino acids.............................................................................7.5g
Sodium citrate...............................................................................3.0g
KCl................................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................1.0g
FeSO 4 ·7H 2 O ...............................................................................0.05g
MnSO 4 ·4H 2 O...........................................................................0.25mg
Preparation of Solution A: Add components, except NaCl, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Add 200.0g of NaCl. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Solution B:
Composition per 100.0mL:
Na 2 CO 3 .........................................................................................5.0g
Preparation of Solution B: Dissolve 5.0g of Na 2 CO 3 in distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. 

Preparation of Medium: Aseptically mix 900.0mL of solution A and 100.0mL of solution B. Mix thoroughly. Aseptically adjust pH to 8.5–9.5. Pour into sterile Petri dishes or distribute into sterile tubes. 

Use: For the cultivation and maintenance of Natronobacterium gregoryi, Natronobacterium magadii, Natronobacterium pharaonis, and Natronococcus occultus.