Haloarcula Medium

[所属分类:培养基配方] [发布时间:2021-3-24] [发布人: ] [阅读次数:] [返回]

Haloarcula Medium

培养基配方

Composition per 1001.0mL:
NaCl..........................................................................................250.0g
MgSO 4 ·7H 2 O..............................................................................20.0g
Agar............................................................................................15.0g

Sodium citrate...............................................................................3.0g

KCl................................................................................................2.0g
CaCl 2 .............................................................................................0.2g
Peptone solution.....................................................................100.0mL
Trace elements solution .............................................................1.0mL
pH 7.4 ± 0.1 at 25°C
Peptone Solution:
Composition per 100.0mL:
Peptone........................................................................................10.0g
Preparation of Peptone Solution: Add peptone to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Trace Elements Solution:
Composition per 100.0mL:
FeCl 2 ·4H 2 O.................................................................................0.36g
MnCl 2 ·4H 2 O..............................................................................0.022g
Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except peptone solution and trace elements solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.4 with NaOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL of sterile peptone solution and 1.0mL of sterile trace elements solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Haloanaerobium praevalens.