Haloarcula vallismortis Synthetic Medium

[所属分类:培养基配方] [发布时间:2021-3-24] [发布人: ] [阅读次数:] [返回]

Haloarcula vallismortis Synthetic Medium

培养基配方

Composition per 1029.0mL:
Basal salts solution........................................................................1.0L
Glucose solution ......................................................................20.0mL
NH 4 Cl solution...........................................................................5.0mL
FeSO 4 ·6H 2 O solution.................................................................2.0mL
K 2 HPO 4 solution........................................................................2.0mL
pH 7.5 ± 0.2 at 25°C
Basal Salts Solution:
Composition per liter:
NaCl..........................................................................................200.0g
MgSO 4 ·7H 2 O..............................................................................36.0g
Tris[hydroxymethyl]aminomethane..............................................6.0g
KCl................................................................................................4.0g
CaCl 2 ·2H 2 O...................................................................................1.0g
Preparation of Basal Salts Solution : Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5 with HCl. Autoclave for 15 min at 15 psi pressure–121°C.
FeSO 4 ·6H 2 O Solution:
Composition per 100.0mL:
FeSO 4 ·6H 2 O..................................................................................0.2g
HCl (1.0mM solution)............................................................100.0mL
Preparation of FeSO 4 ·6H 2 O Solution:  Combine components.
Mix thoroughly. Filter sterilize.
K 2 HPO 4 Solution:
Composition per 100.0mL:
K 2 HPO 4 .........................................................................................5.0g
Preparation of K 2 HPO 4 Solution:  Combine components. Mix thoroughly. Filter sterilize.

NH 4 Cl Solution:
Composition per 100.0mL:
NH 4 Cl .........................................................................................20.0g
Preparation of NH 4 Cl Solution:  Combine components. Mix thoroughly. Filter sterilize.
Glucose Solution:
Composition per 100.0mL:
D -Glucose....................................................................................25.0g
Preparation of Glucose Solution:  Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium:  Aseptically combine 1.0L of sterile basal salts solution with 20.0mL of sterile glucose solution, 5.0mL of sterile NH 4 Cl solution, 2.0mL of sterile K 2 HPO 4 solution, and 2.0mL of sterile FeSO 4 ·6H 2 O solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation of Haloarcula vallismortis.