Halobacteria Agar

[所属分类:培养基配方] [发布时间:2021-3-24] [发布人: ] [阅读次数:] [返回]

Halobacteria Agar

培养基配方

Composition per liter:
NaCl..........................................................................................200.0g
Agar............................................................................................20.0g
MgSO 4 ·7H 2 O..............................................................................20.0g
Casamino acids.............................................................................5.0g
Yeast extract..................................................................................5.0g
Trisodium citrate...........................................................................3.0g
KCl................................................................................................2.0g
Sodium glutamate.........................................................................1.0g
FeCl 2 ·4H 2 O..............................................................................36.0mg
MnCl 2 ·4H 2 O ............................................................................0.36mg
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Haloarcula species, Halobacterium species, Halococcus morrhuae, and Haloferax species.