Halobacteria Medium (DSMZ Medium 372)

[所属分类:培养基配方] [发布时间:2021-3-24] [发布人: ] [阅读次数:] [返回]

Halobacteria Medium

(DSMZ Medium 372)

培养基配方

Composition per liter:
NaCl..........................................................................................200.0g

MgSO 4 ·7H 2 O..............................................................................20.0g

Agar ............................................................................................20.0g
Yeast extract..................................................................................5.0g
Casamino acids.............................................................................5.0g
Na 3 -citrate.....................................................................................3.0g
KCl................................................................................................2.0g
Na-glutamate.................................................................................1.0g
FeCl 2 ·4H 2 O..............................................................................36.0mg
MnCl 2 ·4H 2 O.............................................................................0.36mg
pH 7.1 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the isolation and cultivation of Halorubrum spp., Haloarcula spp., Haloferax spp., Halococcus spp., Haloterrigena spp., Halogeometricum borinquense, Natrialba spp., and Halomicrobium mukohataei.