Halobacteria Medium

[所属分类:培养基配方] [发布时间:2021-3-24] [发布人: ] [阅读次数:] [返回]

Halobacteria Medium

培养基配方

Composition per liter:
NaCl..........................................................................................220.0g
Agar ............................................................................................10.0g
MgSO 4 ·7H 2 O..............................................................................10.0g
Casein hydrolysate........................................................................5.0g
KCl................................................................................................5.0g
Disodium citrate............................................................................3.0g
KNO 3 ............................................................................................1.0g
Yeast extract..................................................................................1.0g
CaCl 2 ·6H 2 O...................................................................................0.2g
pH 7.2–7.4 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until dissolved. Adjust pH to 7.2–7.4. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and enumeration of halobacteria.