Halobacterium halobium/ Halobacterium salinarium Medium

[所属分类:培养基配方] [发布时间:2021-3-25] [发布人: ] [阅读次数:] [返回]

Halobacterium halobium/

Halobacterium salinarium Medium

培养基配方

Composition per liter:
NaCl..........................................................................................250.0g
MgSO 4 ·7H 2 O..............................................................................20.0g
Trisodium citrate·2H 2 O.................................................................3.0g
KCl................................................................................................2.0g
Peptone..........................................................................................2.0g
Trace metals solution.................................................................0.1mL
Trace Metals Solution:
Composition per liter:
ZnSO 4 ·7H 2 O...............................................................................1.32g
Fe(NH 4 ) 2 SO 4 ·7H 2 O ....................................................................0.78g
MnSO 4 ·H 2 O................................................................................0.34g
CuSO 4 ·5H 2 O...............................................................................0.14g
Preparation of Trace Metals Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of Halobacterium halobium and Halobacterium salinarium.