Halobacterium/Halococcus Medium

[所属分类:培养基配方] [发布时间:2021-3-25] [发布人: ] [阅读次数:] [返回]

Halobacterium/Halococcus Medium

培养基配方

Composition per liter:
Solution A..............................................................................500.0mL
Salt solution ...........................................................................500.0mL
Solution A:
Composition per 500.0mL:
Skim milk....................................................................................50.0g
Preparation of Solution A: Add 50.0g of skim milk to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly.

Salt Solution:
Composition per 500.0mL:
MgSO 4 ·7H 2 O..............................................................................10.0g
KNO 3 ............................................................................................2.0g
Preparation of Salt Solution: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. 

Use: For the cultivation of Haloarcula vallismortis, Halobacterium cutirubrum, Halobacterium halobium, Halobacterium saccharovorum, Halobacterium salinarium, and Halococcus morrhuae.