Halobacterium Medium (ATCC Medium 213)

[所属分类:培养基配方] [发布时间:2021-3-25] [发布人: ] [阅读次数:] [返回]

Halobacterium Medium

(ATCC Medium 213)

培养基配方

Composition per liter:
Solution 1...............................................................................500.0mL
Solution 2...............................................................................500.0mL
pH 7.0 ± 0.2 at 25°C
Solution 1:
Composition per 500.0mL:
Yeast extract................................................................................10.0g
Pancreatic digest of casein............................................................2.5g
Preparation of Solution 1: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.0. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Solution 2:
Composition per 500.0mL:
NaCl..........................................................................................250.0g
Agar ............................................................................................20.0g
MgSO 4 ·7H 2 O..............................................................................10.0g
KCl................................................................................................5.0g
CaCl 2 ·6H 2 O...................................................................................0.2g
Preparation of Solution 2: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Coolto 45°–50°C.
Preparation of Medium: Aseptically combine sterile solution 1 and sterile solution 2. Mix thoroughly. Aseptically pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation of Halobacterium salinarium.