Halobacterium Medium (ATCC Medium 974)

[所属分类:培养基配方] [发布时间:2021-3-25] [发布人: ] [阅读次数:] [返回]

Halobacterium Medium

(ATCC Medium 974)

培养基配方

Composition per 100.0mL:
Solution 1.................................................................................75.0mL
Solution 2.................................................................................25.0mL
pH 6.8 ± 0.2 at 25°C
Solution 1:
Composition per 75.0mL:
NaCl............................................................................................12.5g
MgCl 2 ·6H 2 O..................................................................................5.0g
K 2 SO 4 ............................................................................................0.5g
CaCl 2 ·6H 2 O.................................................................................0.02g
Preparation of Solution 1: Add components to distilled/deionized water and bring volume to 75.0mL. Mix thoroughly. Adjust pH to 6.8. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Solution 2:
Composition per 25.0mL:
Agar..............................................................................................2.0g
Pancreatic digest of casein............................................................0.5g
Yeast extract..................................................................................0.5g
Preparation of Solution 2: Add components to distilled/deionized water and bring volume to 25.0mL. Mix thoroughly. Adjust pH to 6.8. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. 

Preparation of Medium: Aseptically combine sterile solution 1 and sterile solution 2. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Haloferax volcanii.