Haloferax volcanii Minimal Medium

[所属分类:培养基配方] [发布时间:2021-3-26] [发布人: ] [阅读次数:] [返回]

Haloferax volcanii Minimal Medium

培养基配方

Composition per liter:
NaCl..........................................................................................206.0g
MgSO 4 ·7H 2 O..............................................................................36.9g
Glycerol ...................................................................................45.0mL
Sodium succinate solution.........................................................5.0mL
KCl solution...............................................................................5.0mL
CaCl 2 ·2H 2 O solution..................................................................5.0mL
NH 4 Cl solution...........................................................................5.0mL
MnCl 2 ·6H 2 O solution.................................................................1.7mL
K 2 HPO 4 solution........................................................................2.0mL
Trace elements solution .............................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Sodium Succinate Solution:
Composition per 100.0mL:
Sodium succinate........................................................................10.0g
Preparation of Sodium Succinate Solution:  Add sodium succinate to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
KCl Solution:
Composition per 100.0mL:
KCl..............................................................................................7.45g
Preparation of KCl Solution:  Add KCl to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
CaCl 2 ·2H 2 O Solution:
Composition per 100.0mL:
CaCl 2 ·2H 2 O.................................................................................10.0g
Preparation of CaCl 2 ·2H 2 O Solution:  Add CaCl 2 ·2H 2 O to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
NH 4 Cl Solution:
Composition per 100.0mL:
NH 4 Cl .........................................................................................5.35g
Preparation of NH 4 Cl Solution:  Add NH 4 Cl to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
MnCl 2 ·6H 2 O Solution:
Composition per liter:
MnCl 2 ·6H 2 O.............................................................................75.0mg
Preparation of MnCl 2 ·6H 2 O Solution:  Add MnCl 2 ·6H 2 O to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

K 2 HPO 4 Solution:
Composition per 100.0mL:
K 2 HPO 4 .........................................................................................8.7g
Preparation of K 2 HPO 4 Solution:  Add K 2 HPO 4 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 7.0.
Trace Elements Solution:
Composition per 100.0mL:
FeSO 4 .....................................................................................334.0mg
MnCl 2 .......................................................................................36.0mg
ZnSO 4 ......................................................................................44.0mg
CuSO 4 ........................................................................................5.0mg
Preparation of Trace Elements Solution:  Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components, except CaCl 2 solution, to distilled/deionized water and bring volume to 995.0mL. Mix thoroughly. Adjust pH to 7.2. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 5.0mL of sterile CaCl 2 solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation of Haloferax volcanii.