Halophilic Halobacterium Medium

[所属分类:培养基配方] [发布时间:2021-3-30] [发布人: ] [阅读次数:] [返回]

Halophilic Halobacterium Medium

培养基配方

Composition per liter:
NaCl..........................................................................................200.0g
MgSO 4 ·7H 2 O..............................................................................37.0g
CaCl 2 ·2H 2 O...................................................................................0.7g
KCl................................................................................................0.5g
MnCl 2 ·4H 2 O................................................................................0.05g
Yeast extract...........................................................................100.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until dissolved. Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of extremely halophilic Halobacterium species.