Halothiobacillus Medium (DSMZ Medium 864)

[所属分类:培养基配方] [发布时间:2021-3-30] [发布人: ] [阅读次数:] [返回]

Halothiobacillus Medium

(DSMZ Medium 864)

培养基配方

Composition per 1030.0mL:
NaCl............................................................................................29.0g
MgSO 4 ·7H 2 O................................................................................1.5g
(NH 4 ) 2 SO 4 .....................................................................................1.0g
KCl................................................................................................0.7g
CaCl 2 ·2H 2 O.................................................................................0.42g
Bromthymol Blue ......................................................................4.0mg
Na-thiosulfate solution.............................................................20.0mL
Phosphate solution...................................................................10.0mL
Trace elements solution .............................................................1.0mL
pH 6.5–7.0 at 25°C
Trace Elements Solution:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
Na 2 -EDTA.....................................................................................0.5g
CoCl 2 ·6H 2 O ...........................................................................190.0mg
MnCl 2 ·4H 2 O...........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
Na 2 MoO 4 ·2H 2 O .......................................................................36.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
H 3 BO 3 ........................................................................................6.0mg
CuCl 2 ·2H 2 O ...............................................................................2.0mg
HCl (25% solution)..................................................................10.0mL
Preparation of Trace Elements Solution: Add FeCl 2 ·4H 2 O to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Adjust pH to 7.0. Sparge with 80% N 2 + 20% CO 2 . Autoclave for 15 min at 15 psi pressure–121°C.
Na-thiosulfate Solution:
Composition per 20.0mL:
Na 2 S 2 O 3 ·5H 2 O..............................................................................5.0g
Preparation of Na-thiosulfate Solution: Add Na 2 S 2 O 3 ·5H 2 O to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Sparge with 100% N 2 . Filter sterilize.
Phosphate Solution:
Composition per 10.0mL:
K 2 HPO 4 .........................................................................................0.5g
Preparation of Phosphate Solution: Add K 2 HPO 4 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N 2 . Filter sterilize.
Preparation of Medium: Add components, except phosphate solution and Na-thiosulfate solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.7. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically add 20.0mL sterile Na-thiosulfate solution and 10.0mL sterile phosphate solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation of Halothiobacillus kellyi.