Halovibrio Medium (DSMZ Medium 1176)

[所属分类:培养基配方] [发布时间:2021-3-30] [发布人: ] [阅读次数:] [返回]

Halovibrio Medium

(DSMZ Medium 1176)

培养基配方

Composition per liter:
NaCl...............................................................................120.0–240.0g
KH 2 PO 4 ........................................................................................3.0g
NH 4 Cl ..........................................................................................0.5g
Sodium acetate soltuion...........................................................10.0mL
HEPES solution.......................................................................10.0mL
Yeast extract solution.................................................................2.0mL
Magnesium sulfate solution.......................................................2.0mL
Trace elements solution SL-6....................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O .................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
HEPES Solution:
Composition per 10.0mL:
HEPES..........................................................................................2.4g
Preparation of HEPES Solution:  Add HEPES to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sodium Acetate Solution:
Composition per 10.0mL:
Sodium acetate............................................................................1.64g
Preparation of Sodium Acetate Solution:  Add sodium acetate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Magnesium Sulfate Solution:
Composition per 10.0mL:
MgSO 4 · 7H 2 O................................................................................2.5g
Preparation of Magnesium Sulfate Solution:  Add MgSO 4 · 7H 2 O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Yeast Extract Solution:
Composition per 10.0mL:
Yeast extract..................................................................................1.0g
Preparation of Yeast Extract Solution:  Add yeast extract to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except yeast extract, magnesium sulfate, and sodium acetate solutions, to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Adjust pH to 7.5. Gently heat while stirring and bring to boiling. Cool to room temperature. Dispense into sealed tubes, vials, or bottles. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically add yeast extract, magnesium sulfate, and sodium acetate solutions.
Use: For the cultivation of Halovibrio spp.