Halovibrio variabilis Medium

[所属分类:培养基配方] [发布时间:2021-3-30] [发布人: ] [阅读次数:] [返回]

Halovibrio variabilis Medium

培养基配方

Composition per liter:
NaCl............................................................................................95.0g
MgSO 4 ·7H 2 O..............................................................................81.0g
Yeast extract..................................................................................7.5g
Proteose peptone...........................................................................2.5g
KCl................................................................................................1.0g
Trace elements solution SL-4 ..................................................10.0mL
Vitamin solution.......................................................................10.0mL
pH 7.5 ± 0.2 at 25°C
Vitamin Solution:
Composition per liter:
Pyridoxine HCl..........................................................................1.0mg
Lipoic acid .................................................................................0.5mg
Nicotinic acid.............................................................................0.5mg
p-Aminobenzoic acid.................................................................0.5mg
Pantothenic acid.........................................................................0.5mg
Riboflavin ..................................................................................0.5mg
Thiamine HCl ............................................................................0.5mg
Biotin .........................................................................................0.2mg
Folic acid....................................................................................0.2mg
Cyanocobalamin ......................................................................0.01mg
Preparation of Vitamin Solution:  Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Store at 5°C.
Trace Elements Solution SL-4:
Composition per 900.0mL:
EDTA............................................................................................0.5g
FeSO 4 ·7H 2 O .................................................................................0.2g
Trace elements solution SL-6 ................................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O ...............................................................................0.03g
CuCl 2 ·2H 2 O................................................................................0.01g
NiCl 2 ·6H 2 O.................................................................................0.02g
Na 2 MoO 4 ·2H 2 O..........................................................................0.03g

Preparation of Trace Elements Solution SL-6: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Trace Elements Solution SL-4:  Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except trace elements solution SL-4 and vitamin solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 10.0mL of sterile trace elements solution SL-4 and 10.0mL of sterile vitamin solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation of Halovibrio variabilis.