Ham’s F-10 Medium

[所属分类:培养基配方] [发布时间:2021-3-30] [发布人: ] [阅读次数:] [返回]

Ham’s F-10 Medium

培养基配方

Composition per liter:
NaCl..............................................................................................7.4g
NaHCO 3 ........................................................................................1.2g
Glucose .........................................................................................1.1g
NaH 2 PO 4 ·H 2 O.............................................................................0.29g
KCl..............................................................................................0.28g
L- Arginine·HCl............................................................................0.21g
L- Glutamine ................................................................................0.15g
MgSO 4 ·7H 2 O..............................................................................0.15g
Sodium pyruvate......................................................................... 0.11g
KH 2 PO 4 .......................................................................................0.08g
CaCl 2 ·2H 2 O ................................................................................0.04g
L- Cystine·2HCl ...........................................................................0.04g
L- Histidine·HCl·H 2 O...................................................................0.02g
L- Lysine·HCl...............................................................................0.02g
L- Asparagine-H 2 O.......................................................................0.01g
L- Aspartic acid............................................................................0.01g
L- Glutamic acid...........................................................................0.01g
L- Leucine ....................................................................................0.01g
L- Proline......................................................................................0.01g
L- Serine.......................................................................................0.01g
L- Alanine....................................................................................8.9mg
Glycine.......................................................................................7.5mg
D- Phenylalanine .........................................................................5.0mg
L- Methionine..............................................................................4.5mg
Hypoxanthine.............................................................................4.1mg
L- Threonine................................................................................3.6mg
L- Valine..................................................................................... 3.5mg
L- Isoleucine................................................................................2.6mg
L- Tyrosine ..................................................................................1.8mg
Vitamin B 12 ................................................................................1.4mg
Folic acid ...................................................................................1.3mg
Phenol Red.................................................................................1.2mg
Thiamine·HCl............................................................................1.0mg
FeSO 4 ·7H 2 O...............................................................................0.8mg
Choline chloride.........................................................................0.7mg
D- Calcium pantothenate.............................................................0.7mg
Thymidine..................................................................................0.7mg
Niacinamide...............................................................................0.6mg
L- Tryptophan..............................................................................0.6mg
Isoinositol ..................................................................................0.5mg
Riboflavin..................................................................................0.4mg
Lipoic acid.................................................................................0.2mg
Pyridoxine·HCl..........................................................................0.2mg
ZnSO 4 ·7H 2 O............................................................................0.03mg
Biotin.......................................................................................0.02mg
CuSO 4 ·5H 2 O...............................................................................3.0μg
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.
Use: For the growth of Y-1 cell cultures used in the mouse adrenal assay for heat-labile toxin of enterotoxigenic Escherichia coli and Vibrio species.