Harpo’s HTYEM Marine Medium

[所属分类:培养基配方] [发布时间:2021-3-30] [发布人: ] [阅读次数:] [返回]

Harpo’s HTYEM Marine Medium

培养基配方

Pancreatic digest of casein............................................................5.0g
HEPES..........................................................................................4.0g
Yeast extract..................................................................................2.0g
Artificial seawater.........................................................................1.0L
pH 7.5 ± 0.2 at 25°C
Artificial Seawater:
Composition per liter:
NaCl............................................................................................27.5g
MgSO 4 ·7H 2 O..............................................................................6.78g
MgCl 2 ·6H 2 O................................................................................5.38g
CaCL 2 ·2H 2 O.................................................................................1.4g
KCl..............................................................................................0.72g
NaHCO 3 ....................................................................................... 0.2g
Preparation of Artificial Seawater: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to artificial seawater and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5 with NaOH. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Cytophaga fermentans, Cytophaga latercula, Cytophaga uliginosa, and Microscilla aggregans.