Hartley’s Digest Broth

[所属分类:培养基配方] [发布时间:2021-3-30] [发布人: ] [阅读次数:] [返回]

Hartley’s Digest Broth

培养基配方

Composition per 10.0L:
Ox heart ..................................................................................3000.0g
Pancreatin ...................................................................................50.0g
Na 2 CO 3 , anhydrous (0.8% solution).............................................5.0L
HCl, concentrated....................................................................80.0mL
pH 7.5 ± 0.2 at 25°C
Preparation of Medium: Finely mince the ox heart. Add the meat to 5.0L of distilled/deionized water. Gently heat and bring to 80°C. Add the 5.0L of Na 2 CO 3 solution. Cool to 45°C. Add pancreatin and maintain at 45°C for 4 hr while stirring. Add the HCl and steam at 100°C for 30 min. Cool to room temperature. Adjust pH to 8.0 with 1N NaOH. Gently heat and bring to boiling. Continue boiling for 25 min. Filter while hot through Whatman #1 filter paper. Cool to room temperature. Adjust pH to 7.5. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the isolation and cultivation of Actinobacillus lignieresii from cattle.