Hay Extract Agar (HAY)

[所属分类:培养基配方] [发布时间:2021-3-31] [发布人: ] [阅读次数:] [返回]

Hay Extract Agar

(HAY)

培养基配方

Composition per liter:
Hay..............................................................................................50.0g
Agar ............................................................................................20.0g
pH 6.2 ± 0.2 at 25°C
Preparation of Medium: Add finely hashed hay to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 30 min at 15 psi pressure–121°C. Filter through Whatman filter paper. Adjust pH of filtrate to 6.2 with 5% potassium phosphate solution. Add agar. Bring volume to 1.0L wtih distilled/deionized water. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 20 min at 10 psi pressure–115°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation of Alternaria porri, Botryotinia draytonii, Botryotinia fuckeliana, Botryotinia porri, Botryotinia narcissicola, Botryotinia polyblastis, Botrytis aclada, Botrytis hyacinthi, Botrytis cinerea, Cercospora beticola, and Sclerotinia sphaerosperma.