Hay Infusion Agar

[所属分类:培养基配方] [发布时间:2021-3-31] [发布人: ] [阅读次数:] [返回]

Hay Infusion Agar

培养基配方

Composition per liter:
Hay, partially decomposed..........................................................50.0g
Agar ............................................................................................15.0g
K 2 HPO 4 .........................................................................................2.0g
pH 6.2 ± 0.3 at 25°C
Preparation of Medium:  Add hay to distilled/deionized water and bring volume to 1.0L. Autoclave for 30 min at 15 psi pressure–121°C. Filter through paper and reserve filtrate. Add distilled/deionized water to filtrate and bring volume to 1.0L. Mix thoroughly. Add agar and K 2 HPO 4 . Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 6.2. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Alternaria species, Caulochytrium protostelioides, Choanephora infundibulifera, Dipsacomyces acuminosporus, Eremascus albus, Eremascus fertilis, Eurotium chevalieri, Eurotium halophilicum, Eurotium herbariorum, Mortierella bisporalis, Protostelium irregularis, and Saksenaea vasiformis.