Hayflick Medium

[所属分类:培养基配方] [发布时间:2021-3-31] [发布人: ] [阅读次数:] [返回]

Hayflick Medium

培养基配方

Composition per 107.5mL:
Mycoplasma broth base...........................................................70.0mL
Horse serum.............................................................................20.0mL
Fresh yeast extract solution......................................................10.0mL
Penicillin solution......................................................................5.0mL
Thallous acetate solution ...........................................................2.5mL
pH 7.8 ± 0.2 at 25°C
Mycoplasma Broth Base:
Pancreatic digest of casein............................................................7.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................3.0g
Yeast extract..................................................................................3.0g
Beef heart, solids from infusion....................................................2.0g
pH 7.8 ± 0.2 at 25°C
Preparation of Mycoplasma Broth Base: Add components to distilled/deionized water and bring volume to 1.0L. Gently heat and bring to boiling. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Fresh Yeast Extract Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free......................................25.0g
Preparation of Fresh Yeast Extract Solution : Add live Baker’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90 min at 15 psi pressure–121°C. Allow to stand. Remove supernatant solution. Adjust pH to 6.6–6.8. Filter sterilize.
Penicillin Solution:
Composition per 5.0mL:
Penicillin................................................................................ 20,000U
Preparation of Penicillin Solution: Add penicillin to distilled/deionized water and bring volume to 5.0mL. Mix thoroughly. Filter sterilize.
Thallous Acetate Solution:
Composition per 10.0mL:
Thallous acetate............................................................................0.1g
Preparation of Thallous Acetate Solution: Add thallous acetate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Use: For the cultivation of Mycoplasma species.