HD Agar, 1:10 Diluted, Modified (DSMZ Medium 1135)

[所属分类:培养基配方] [发布时间:2021-3-31] [发布人: ] [阅读次数:] [返回]

HD Agar, 1:10 Diluted, Modified

(DSMZ Medium 1135)

培养基配方

Composition per liter:
MES (2-[N-morpholino]ethane sulfonic acid) ...........................1.95g
Casein peptone .............................................................................0.5g

Yeast extract ...............................................................................0.25g

Glucose .........................................................................................0.1g

Agar solution..........................................................................500.0mL
pH 5.5 ± 0.2 at 25°C
Agar Solution:
Composition per 10.0mL:
Agar ............................................................................................15.0g
Preparation of Agar Solution:  Wash agar with double distilled water. Add agar to double distilled/deionized water and bring volume to 500.0mL. Adjsut pH to 5.5. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Preparation of Medium: Add components, except agar solution, to double distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 5.5. Gently heat while stirring and bring to boiling. Mix thoroughly. Autoclave for 15 min at 15 psi–121°C. Cool to 50°C. Aseptically add 500.0mL agar solution. Pour into Petri dishes or aseptically distribute into tubes.
Use: For the cultivation of Edaphobacter aggregans.