HD- Medium, 1:10 Diluted, Modified (DSMZ Medium 1135)

[所属分类:培养基配方] [发布时间:2021-3-31] [发布人: ] [阅读次数:] [返回]

HD- Medium, 1:10 Diluted, Modified

(DSMZ Medium 1135)

培养基配方

Composition per liter:
MES (2-[N-Morpholino]ethane sulfonic acid) ..........................1.95g
Casein peptone .............................................................................0.5g
Yeast extract ...............................................................................0.25g
Glucose ........................................................................................0.1g
pH 5.5 ± 0.2 at 25°C
Preparation of Medium: Add components to double distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.5. Gently heat while stirring and bring to boiling. Mix thoroughly. Distribute into tubes , bottles, or flasks. Autoclave for 15 min at 15 psi pressure–121°C .
Use: For the cultivation of Edaphobacter aggregans.