HE Medium (Hay Extract Medium)

[所属分类:培养基配方] [发布时间:2021-3-31] [发布人: ] [阅读次数:] [返回]

HE Medium

(Hay Extract Medium)

培养基配方

Composition per liter:
Agar ............................................................................................10.0g
Peptone..........................................................................................1.0g
Yeast extract..................................................................................1.0g
Hay extract solution...............................................................500.0mL
pH 6.5 ± 0.2 at 25°C
Hay Extract Solution:
Composition per liter:
Hay, dried....................................................................................50.0g
Preparation of Hay Extract Solution: Add dried barn hay to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Filter through Whatman #40 filter paper. 

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the isolation and cultivation of Spirochaeta aurantia.