Heart Infusion Broth with Horse Serum, Fresh Yeast Extract, and Penicillin

[所属分类:培养基配方] [发布时间:2021-4-1] [发布人: ] [阅读次数:] [返回]

Heart Infusion Broth with Horse Serum, Fresh Yeast

Extract, and Penicillin

培养基配方

Composition per 940.0mL:
Heart infusion broth...............................................................720.0mL
Horse serum, unheated...........................................................200.0mL
Fresh yeast extract solution .....................................................10.0mL
Penicillin solution....................................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Heart Infusion Broth:
Composition per liter:
Beef heart, infusion from..........................................................500.0g
Tryptose......................................................................................10.0g
NaCl..............................................................................................5.0g
Preparation of Heart Infusion Broth:  Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Fresh Yeast Extract Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free......................................25.0g
Preparation of Fresh Yeast Extract Solution : Add the live Baker’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90 min at 15 psi pressure–121°C. Allow to stand. Remove supernatant solution. Adjust pH to 6.6–6.8.
Penicillin Solution:
Composition per 10.0mL:
Penicillin.............................................................................. 100,000U
Preparation of Penicillin Solution: Add penicillin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium:  To 720.0mL of sterile cooled heart infusion broth, aseptically add 200.0mL of horse serum, 10.0mL of fresh yeast extract solution, and 10.0mL of sterile penicillin solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Mycoplasma equigenitalium and Mycoplasma subdolum.