Heart Infusion Broth with Human Serum and Fresh Yeast Extract

[所属分类:培养基配方] [发布时间:2021-4-1] [发布人: ] [阅读次数:] [返回]

Heart Infusion Broth with Human

Serum and Fresh Yeast Extract

培养基配方

Composition per 930.0mL:
Heart infusion broth...............................................................720.0mL
Human serum, unheated........................................................200.0mL
Fresh yeast extract solution .....................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Heart Infusion Broth:
Composition per liter:
Beef heart, infusion from..........................................................500.0g
Tryptose......................................................................................10.0g
NaCl..............................................................................................5.0g
Preparation of Heart Infusion Broth:  Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Fresh Yeast Extract Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free......................................25.0g
Preparation of Fresh Yeast Extract Solution : Add the live Baker’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90 min at 15 psi pressure–121°C. Allow to stand. Remove supernatant solution. Adjust pH to 6.6–6.8.

Preparation of Medium:  To 720.0mL of sterile, cooled heart infusion broth, aseptically add 200.0mL of human serum and 10.0mL of fresh yeast extract solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Mycoplasma equigenitalium and Mycoplasma subdolum.