Heart Infusion Broth with Inactivated Horse Serum

[所属分类:培养基配方] [发布时间:2021-4-1] [发布人: ] [阅读次数:] [返回]

Heart Infusion Broth

with Inactivated Horse Serum

培养基配方

Composition per liter:
Beef heart, infusion from..........................................................500.0g
Tryptose ......................................................................................10.0g
NaCl..............................................................................................5.0g
Horse serum, inactivated........................................................100.0mL
pH 7.6 ± 0.2 at 25°C
Preparation of Medium: Add components, except horse serum, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile horse serum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Enterococcus faecium.