Heart Infusion Broth with Inactivated Horse Serum, Fresh Yeast Extract, and Sucrose

[所属分类:培养基配方] [发布时间:2021-4-1] [发布人: ] [阅读次数:] [返回]

Heart Infusion Broth with Inactivated Horse Serum,

Fresh Yeast Extract, and Sucrose

培养基配方

Composition per liter:
Beef heart, infusion from..........................................................500.0g
Sucrose........................................................................................40.0g
Tryptose ......................................................................................10.0g
NaCl..............................................................................................5.0g
Horse serum, inactivated .......................................................200.0mL
Fresh yeast extract solution ...................................................100.0mL
pH 7.5 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Diagnostic Systems.
Fresh Yeast Extract Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free......................................25.0g
Preparation of Fresh Yeast Extract Solution : Add the live Baker’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90 min at 15 psi pressure–121°C. Allow to stand. Remove supernatant solution. Adjust pH to 6.6–6.8.
Preparation of Medium: Add components, except horse serum and fresh yeast extract solution, to distilled/deionized water and bring volume to 700.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile horse serum and fresh yeast extract solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Acholeplasma species, Mycoplasma species, and Streptobacillus species.