Heart Infusion Broth (pH 7.5) with Inactivated Human Serum and Yeast Extract (ATCC Medium 245)

[所属分类:培养基配方] [发布时间:2021-4-1] [发布人: ] [阅读次数:] [返回]

Heart Infusion Broth (pH 7.5) with Inactivated
Human Serum and Yeast Extract

(ATCC Medium 245)

培养基配方

Composition per liter:
Heart infusion broth with yeast extract..................................800.0mL
Human serum, inactivated.....................................................200.0mL
pH 7.5 ± 0.2 at 25°C
Heart Infusion Broth with Yeast Extract:
Composition per liter:
Beef heart, infusion from..........................................................500.0g
Tryptose......................................................................................10.0g
Yeast extract (Oxoid)....................................................................6.3g
NaCl..............................................................................................5.0g

Preparation of Heart Infusion Broth with Yeast Extract:

Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°.

Source:  Heart infusion broth without yeast extract is available as a premixed powder from BD Diagnostic Systems.
Preparation of Medium:  To 800.0mL of sterile cooled heart infusion broth with yeast extract, aseptically add 200.0mL of heat inactivated human serum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Corynebacterium pseudotuberculosis and Streptobacillus moniliformis.