Helicobacter Medium

[所属分类:培养基配方] [发布时间:2021-4-2] [发布人: ] [阅读次数:] [返回]

Helicobacter Medium

培养基配方

Composition per 850.0mL:
Agar ............................................................................................15.0g
Beef extract.................................................................................10.0g
Peptone........................................................................................10.0g
NaCl..............................................................................................5.0g
Horse blood, laked...................................................................50.0mL
Antibiotic solution A ...............................................................10.0mL
Antibiotic solution B................................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Antibiotic Solution A:
Composition per 10.0mL:
Vancomycin...............................................................................1.0mg
Trimethoprim.............................................................................5.0mg
Polymyxin B.............................................................................. 250 U
Preparation of Antibiotic Solution A: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Antibiotic Solution B:
Composition per 10.0mL:
Amphotericin B .........................................................................5.0mg
Dimethylformamide...................................................................2.0mL
Preparation of Antibiotic Solution B: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except laked horse blood, antibiotic solution A, and antibiotic solution B, to distilled/deionized water and bring volume to 930.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 50.0mL of sterile laked horse blood, 10.0mL of sterile antibiotic solution A, and 10.0mL of sterile antibiotic solution B. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Helicobacter muridarum and Helicobacter felis.