Helicobacter pylori Isolation Agar

[所属分类:培养基配方] [发布时间:2021-4-2] [发布人: ] [阅读次数:] [返回]

Helicobacter pylori Isolation Agar

培养基配方

Composition per liter:
Agar............................................................................................15.0g
Bitone..........................................................................................10.0g
Pancreatic digest of casein............................................................5.0g
NaCl..............................................................................................5.0g
Peptic digest of animal tissue .......................................................5.0g
Tryptic digest of beef heart...........................................................3.0g
Cornstarch.....................................................................................1.0g
Horse blood, laked...................................................................35.0mL
Antibiotic inhibitor solution ....................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Antibiotic Inhibitor Solution:
Composition per 10.0mL:
Vancomycin................................................................................0.01g
Amphotericin B .........................................................................5.0mg
Cefsulodin..................................................................................5.0mg
Trimethoprim lactate..................................................................5.0mg
Preparation of Antibiotic Inhibitor Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except horse blood and antibiotic inhibitor solution, to distilled/deionized water and bring volume to 955.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile horse blood and sterile antibiotic inhibitor solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Helicobacter pylori from clinical specimens.