Helicobacter pylori Selective Medium

[所属分类:培养基配方] [发布时间:2021-4-2] [发布人: ] [阅读次数:] [返回]

Helicobacter pylori Selective Medium

培养基配方

Composition per 1080.0mL:
Special peptone...........................................................................23.0g
Agar ............................................................................................10.0g
NaCl..............................................................................................5.0g
Starch ............................................................................................1.0g
Horse blood, laked...................................................................70.0mL
Selective supplement solution .................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid Unipath.
Horse Blood, Laked:
Composition per 100.0mL:
Horse blood, fresh..................................................................100.0mL
Preparation of Horse Blood, Laked: Add blood to a sterile polypropylene bottle. Freeze overnight at −20°C. Thaw at 8°C. Refreeze at −20°C. Thaw again at 8°C.
Selective Supplement Solution:
Composition per 10.0mL:
Vancomycin .............................................................................10.0mg
Trimethoprim.............................................................................5.0mg
Cefsulodin..................................................................................5.0mg
Amphotericin B..........................................................................5.0mg
Preparation of Selective Supplement Solution:  Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except selective supplement solution and laked horse blood, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptially add 10.0mL selective supplement solution and 70.0mL sterile laked horse blood. Mix thoroughly. Pour into sterile Petri dishes.
Use:  For the isolation of Helicobacter pylori from clinical specimens. H. pylori forms discrete, translucent, and non-coalescent colonies.