Heliobacillus mobilis Medium

[所属分类:培养基配方] [发布时间:2021-4-2] [发布人: ] [阅读次数:] [返回]

Heliobacillus mobilis Medium

培养基配方

Composition per 966.0mL:
Yeast extract................................................................................10.0g
MgSO 4 ..........................................................................................0.1g
EDTA.........................................................................................2.0mg
Sodium pyruvate solution......................................................100.0mL
Trace elements solution B........................................................10.0mL
K 2 HPO 4  solution........................................................................5.0mL
Trace elements solution A..........................................................1.0mL
pH 7.1 ± 0.2 at 25°C
Sodium Pyruvate Solution:
Composition per 100.0mL:
Sodium pyruvate...........................................................................1.1g
Preparation of Sodium Pyruvate Solution: Add sodium pyruvate to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Trace Elements Solution B:
Composition per 100.0mL:
CaCl 2 ·2H 2 O...................................................................................0.3g
Ferric ammonium citrate...............................................................0.2g
Preparation of Trace Elements Solution B:  Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
K 2 HPO 4 Solution:
Composition per 100.0mL:
K 2 HPO 4 .........................................................................................4.0g
Preparation of K 2 HPO 4 Solution: Add K 2 HPO 4 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Trace Elements Solution A:
Composition per 100.0mL:
H 3 BO 3 .........................................................................................2.86g
MnCl 2 ·4H 2 O ...............................................................................1.81g
Na 2 MoO 4 ·2H 2 O..........................................................................0.39g
ZnSO 4 ·7H 2 O.............................................................................0.222g
CuSO 4 ·5H 2 O.............................................................................0.079g
Co(NO 3 ) 2 ·6H 2 O.......................................................................49.4mg
Preparation of Trace Elements Solution A:  Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except sodium pyruvate solution, trace elements solution B, K 2 HPO 4  solution, and trace elements solution A, to distilled/deionized water and bring volume to 850.0mL. Mix thoroughly. Adjust pH to 7.1. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically add 100.0mL of sterile sodium pyruvate solution, 10.0mL of sterile trace elements solution B, 5.0mL of sterile K 2 HPO 4  solution, and 1.0mL of sterile trace elements solution A. Mix thoroughly. Aseptically distibute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Heliobacillus mobilis.