Heliobacterium chlorum Medium

[所属分类:培养基配方] [发布时间:2021-4-2] [发布人: ] [阅读次数:] [返回]

Heliobacterium chlorum Medium

培养基配方

Composition per liter:
Yeast extract................................................................................10.0g
K 2 HPO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................1.0g
Sodium ascorbate..........................................................................0.5g
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components, except sodium ascorbate, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Sparge with 100% N 2 and continue boiling for 3–4 min. Add sodium ascorbate and continue to sparge with 100% N 2 . Adjust pH to 6.8. Under 100% N 2 , immediately dispense 45.0mL of medium into 50.0mL screw-capped tubes fitted with rubber septa. Tighten screw caps. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and maintenance of Heliobacillus mobilis and Heliobacterium chlorum.