Heliorestis Medium (DSMZ Medium 886)

[所属分类:培养基配方] [发布时间:2021-4-2] [发布人: ] [阅读次数:] [返回]

Heliorestis Medium

(DSMZ Medium 886)

培养基配方

Composition per liter:
Na-acetate.....................................................................................1.0g
MgCl 2 ·6H 2 O .................................................................................0.6g
Yeast extract..................................................................................0.5g
KH 2 PO 4 .........................................................................................0.5g
NaCl..............................................................................................0.5g
Resazurin......................................................................................0.2g
CaCl 2 .............................................................................................0.1g
Vitamin  B 12 ...............................................................................20.0μg
Biotin ........................................................................................20.0μg
Solution A................................................................................50.0mL
Trace elements solution SL-6 ....................................................1.0mL
pH 9.0–9.5 at 25°C
Solution A :
Composition per 50.0mL:
Na 2 CO 3 .........................................................................................2.5g
NaHCO 3 ........................................................................................2.5g
NH 4 Cl ...........................................................................................0.5g
Na 2 S·9H 2 O....................................................................................0.4g
Preparation of Solution A: Add components to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O..................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O ......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O ................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. 

Preparation of Medium:  Add components, except solution A, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 50.0mL solution A. Mix thoroughly. Adjust pH to 9.0–9.5. Aseptically distribute to sterile tubes or flasks.

Use: For the cultivation of Heliorestis baculata.