Hemmes Medium Base

[所属分类:培养基配方] [发布时间:2021-4-2] [发布人: ] [阅读次数:] [返回]

Hemmes Medium Base

培养基配方

Composition per liter:
Casein enzymatic hydrolysate ....................................................10.0g
Lactose........................................................................................10.0g
Sucrose........................................................................................10.0g
Agar ..............................................................................................5.5g
NaCl..............................................................................................4.0g
Yeast extract..................................................................................3.0g
Glucose .........................................................................................0.3g
Na 2 S 2 O 3 ·5H 2 O..............................................................................0.1g
FeSO 4 ·7H 2 O................................................................................0.04g
Phenol Red................................................................................0.015g
Urea solution..............................................................................5.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Urea Solution:
Composition per 10.0mL:
Urea...............................................................................................4.0g
Preparation of Urea Solution:  Add urea to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except selective supplement solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add selective supplement solution. Mix thoroughly. Pour into Petri dishes or aseptically distribute into sterile tubes.
Use: For the detection of Salmonella spp. and Shigella spp. based upon 7 different metabolic reactions.