Hemo ID Quad Plate with Growth Factors (Haemophilus Identification Quadrant Plate with Growth Factors)

[所属分类:培养基配方] [发布时间:2021-4-2] [发布人: ] [阅读次数:] [返回]

Hemo ID Quad Plate with Growth Factors
(Haemophilus Identification Quadrant Plate

with Growth Factors)

培养基配方

Composition per plate:
Quadrant I..................................................................................5.0mL
Quadrant II.................................................................................5.0mL
Quadrant III ...............................................................................5.0mL
Quadrant IV...............................................................................5.0mL
Quadrant I:
Composition per 5.0mL:
Hemin ........................................................................................0.1mg
Brain heart infusion agar ...........................................................5.0mL
Quadrant II:
Composition per 5.0mL:
Brain heart infusion agar ...........................................................5.0mL
Supplement solution................................................................0.05mL
Quadrant III:
Composition per 5.0mL:
Hemin ........................................................................................0.1mg
Brain heart infusion agar ...........................................................5.0mL
Supplement solution................................................................0.05mL
Quadrant IV:
Composition per 5.0mL:
Hemin ........................................................................................0.1mg
Brain heart infusion agar ...........................................................5.0mL
Horse blood..............................................................................0.25mL
Supplement solution................................................................0.05mL
Source: The supplement solution (IsoVitaleX ® enrichment) is available from BD Diagnostic Systems. This enrichment may be replaced by supplement VX from BD Diagnostic Systems.
Preparation of Quadrant Media: Sterilize Brain Heart Infusion Agar by autocalving for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Add additional components as filter sterilized solutions. Mix and distribute as 5.0mL aliquots into quadrants.
Use: For the differentiation and presumptive identification of Haemophilus species. The Hemo ID Quad Plate is a four-sectored plate, each with a different medium.