Hemorrhagic Coli Agar with MUG (HC Agar with MUG) (BAM M62)

[所属分类:培养基配方] [发布时间:2021-4-2] [发布人: ] [阅读次数:] [返回]

Hemorrhagic Coli Agar with MUG
(HC Agar with MUG)

(BAM M62)

培养基配方

Composition per liter:
Sorbitol........................................................................................20.0g
Pancreatic digest of casein..........................................................20.0g
Agar ............................................................................................15.0g
NaCl..............................................................................................5.0g
Bile salts No. 3............................................................................1.12g
Bromcresol Purple ....................................................................0.015g
MUG reagent ................................................................................0.1g
pH 7.2 ± 0.2 at 25°C
Source:  MUG reagent is available from Hach Company, Loveland, Colorado.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes.
Use : For the isolation and cultivation of enterohemorraghic Escherichia coli from food. For hemorrhagic colitis E. coli strains.